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Showing 3 results for Besharati

A. Fazaeli, H. Besharati,
Volume 3, Issue 1 (Journal of Science and Technology of Greenhouse Culture 2012)
Abstract

This greenhouse experiment was carried out to evaluate the effects of salinity and bacterial inoculation on some growth indices and total protein content of alfalfa (Medicago sativa) using a factorial completely randomized design with three replications. The effect of three salinity levels (0, 6 and 12 dS/m) induced by a mixture of NaCl, CaCl2 and MgCl2 salts on growth indices and protein content of three alfalfa genotypes (Hamadani, Gharahyonjeh and Gharghalogh) at three levels of inoculation with Sinorhizobium meliloti bacteria (no inoculation, inoculation with salinity-tolerant strain and inoculation with salinity-sensitive strain) was investigated. After the isolation and purification of alfalfa-symbiotic-bacteria from alfalfa fields in Tehran province, two isolates of S. meliloti, one salinity-resistant and the other one salinity-sensitive, which are effective in symbiosis with alfalfa, were selected. Analysis of the results showed that by increasing the salinity level, the shoot and root dry weight, number of active nodules, and nitrogen (N) concentration were decreased significantly (P<0.01). Inoculation with salinity-resistant strain of S. meliloti caused significant increase in shoot and root dry weight, number of active nodules, and N concentration of plants. Moreover, under saline conditions, the salinity-resistant S. meliloti strain increased significantly most alfalfa growth-indices and yield compared to control (without inoculation) and inoculation with salinity-sensitive bacteria. There was no significant difference among alfalfa genotypes in yield and other growth indices under saline conditions. In general, the R59 isolate seems to be the best isolate of S. meliloti for greenhouse-grown alfalfa in saline conditions.
S. Morad, H. Besharati, V. Faizi-Asl, J. Sheikhi,
Volume 7, Issue 2 (Journal of Science and Technology of Greenhouse Culture 2016)
Abstract

To evaluate the effect of arbuscular mycorrhizal (AM) fungi (Glomus mosseae and Glomus intraradices) and Mesorhizobium Ciceri bacteria at three soil moisture levels [28% (field capacity, FC), 15% (-5 bar suction) and 9% (-10 bar tension) by weight] on morphological properties of root and aerial parts of chickpea, a greenhouse factorial experiment, arranged as a complete randomized design, was conducted in a sterilized soil. Results showed that moisture treatment had significance effect on number of pods, number of seeds, fresh and dry weight of root and aerial parts, plant height and root length and volume. Application of Mesorhizobium was effective on number of nodes, number of pods, number of seeds, fresh and dry weight of root and aerial parts, plant height, root length and volume. Glomus mosseae had significant effect on plant height. Interaction of moisture and AM fungi was significant on fresh and dry weight of root and aerial parts. Interaction effect of Mesorhizobium and soil moisture was only significant on number of nodes. In general, the highest fresh and dry weight of aerial parts (44.6 and 10.53 grams, respectively) was obtained by inoculation of chickpea by rhizobium bacteria and Glomus mosseae at FC moisture level, and AM fungi Glomus mosseae was more efficient compared to Glomus intraradices in both drought stress and without stress conditions.


S. Moradi, H. Besharati, V. Feizi Asl, J. Sheikhi,
Volume 8, Issue 2 (Journal of Science and Technology of Greenhouse Culture 2017)
Abstract

Chickpea (Cicer arientinum L.) is one of the Leguminosae family members that has symbiotic ability with Mesorhizobium ciceri bacteria and arbuscular mycorrhizal (AM) fungi. To evaluate the effect of AM fungi (Glomus mosseae, Glomus intraradices) and Mesorhizobium ciceri bacteria at three soil moisture levels [28% (field capacity), 15% (-5 bar suction) and 9% (-10 bar suction)] on nutrients concentration of roots, areal parts of chickpea and post-harvest soil, a greenhouse factorial experiment was conducted, arranged as a completely randomized design, in sterilized soil. Results showed that moisture content had significant effect on potassium (K) concentration of roots, areal parts and post-harvest soil; the highest positive effect of moisture was at field capacity level. Rhizobium bacteria had significant effect on concentration of K and nitrogen (N) of the roots, phosphorus (P) of the areal parts, and post-harvest iron (Fe) concentration in the soil. AM fungi had significant effect on root P concentration of the roots; the highest effect was related to Glomus mosseae. Interaction of moisture and AM fungi was significant on concentration of root P and areal parts manganese (Mn); the highest effect was related to Glomus mosseae treatment at field capacity moisture level. Interaction of moisture and rhizobium bacteria was significant on concentration of root N and P, and areal parts Mn and Fe; the highest effect was related to rhizobium treatment at field capacity moistute level. Interaction of AM fungi and rhizobium bacteria was significant on root P concentration; the highest effect was related to rhizobium and Glomus mosseae treatment. The highest root N concentration was related to combination of AM fungi and inoculation with rhizobium treatment at field capacity moisture level.


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