fa ارزیابی فعالیت آنزیم فسفاتاز قلیایی و قابلیت استفاده شکل‌های مختلف فسفر برای گیاه لوبیا (Phaseolus vulgaris) در تعدادی از خاک‏های آهکی تیمار شده با لجن فاضلاب شهری پژوهشي Research <p align="justify"><strong><span style="FONT-FAMILY: Lotus FONT-SIZE: 11pt mso-fareast-font-family: 'Times New Roman' mso-ansi-language: EN-US mso-fareast-language: EN-US mso-bidi-language: FA mso-ansi-font-size: 9.0pt mso-ascii-font-family: 'Times New Roman Bold' mso-hansi-font-family: 'Times New Roman Bold' mso-no-proof: yes" dir="rtl" lang="FA">برای ارزیابی ارتباط بین اجزای مختلف فسفر و آنزیم فسفاتاز قلیایی با شاخص­های گیاه<font face="Lotus"><span style="mso-spacerun: yes"><font size="3">  </font></span>لوبیا در 10 نوع خاک آهکی تیمار شده با لجن فاضلاب شهری از استان چهارمحال و بختیاری، پژوهشی در گلخانه اجرا شد. در ابتدا، نمونه­های خاک تهیه شده یک ماه با لجن فاضلاب شهری در نرخی معادل با 1% وزنی/وزنی خوابانیده شدند. سپس، شکل­های مختلف فسفر که شامل فسفر پیوند شده به اکسیدهای آهن و آلومینیوم (</font></span><span style="FONT-FAMILY: "Times New Roman Bold","serif" FONT-SIZE: 9pt mso-fareast-font-family: 'Times New Roman' mso-ansi-language: EN-US mso-fareast-language: EN-US mso-bidi-language: AR-SA mso-no-proof: yes mso-bidi-font-size: 11.0pt mso-bidi-font-family: Lotus">NaOH+CB]-P</span><span dir="rtl"></span><span style="FONT-FAMILY: Lotus FONT-SIZE: 11pt mso-fareast-font-family: 'Times New Roman' mso-ansi-language: EN-US mso-fareast-language: EN-US mso-bidi-language: AR-SA mso-ansi-font-size: 9.0pt mso-ascii-font-family: 'Times New Roman Bold' mso-hansi-font-family: 'Times New Roman Bold' mso-no-proof: yes" dir="rtl" lang="AR-SA"><span dir="rtl"></span>]</span><span style="FONT-FAMILY: Lotus FONT-SIZE: 11pt mso-fareast-font-family: 'Times New Roman' mso-ansi-language: EN-US mso-fareast-language: EN-US mso-bidi-language: FA mso-ansi-font-size: 9.0pt mso-ascii-font-family: 'Times New Roman Bold' mso-hansi-font-family: 'Times New Roman Bold' mso-no-proof: yes" dir="rtl" lang="FA">)، فسفر مسدود شده در اکسیدهای آهن (</span><span style="FONT-FAMILY: "Times New Roman Bold","serif" FONT-SIZE: 9pt mso-fareast-font-family: 'Times New Roman' mso-ansi-language: EN-US mso-fareast-language: EN-US mso-bidi-language: FA mso-no-proof: yes mso-bidi-font-size: 11.0pt mso-bidi-font-family: Lotus">CBD-P</span><span dir="rtl"></span><span style="FONT-FAMILY: Lotus FONT-SIZE: 11pt mso-fareast-font-family: 'Times New Roman' mso-ansi-language: EN-US mso-fareast-language: EN-US mso-bidi-language: FA mso-ansi-font-size: 9.0pt mso-ascii-font-family: 'Times New Roman Bold' mso-hansi-font-family: 'Times New Roman Bold' mso-no-proof: yes" dir="rtl" lang="FA"><span dir="rtl"></span>) و فسفر در ارتباط با کلسیم (</span><span style="FONT-FAMILY: "Times New Roman Bold","serif" FONT-SIZE: 9pt mso-fareast-font-family: 'Times New Roman' mso-ansi-language: EN-US mso-fareast-language: EN-US mso-bidi-language: FA mso-no-proof: yes mso-bidi-font-size: 11.0pt mso-bidi-font-family: Lotus">HCl-P</span><span dir="rtl"></span><span style="FONT-FAMILY: Lotus FONT-SIZE: 11pt mso-fareast-font-family: 'Times New Roman' mso-ansi-language: EN-US mso-fareast-language: EN-US mso-bidi-language: FA mso-ansi-font-size: 9.0pt mso-ascii-font-family: 'Times New Roman Bold' mso-hansi-font-family: 'Times New Roman Bold' mso-no-proof: yes" dir="rtl" lang="FA"><span dir="rtl"></span>)، به روش عصاره­گیری مرحله­ای اولسن و سامرز تعیین شد. هم‌چنین، فسفر کل، فسفر آلی (</span><span style="FONT-FAMILY: "Times New Roman Bold","serif" FONT-SIZE: 9pt mso-fareast-font-family: 'Times New Roman' mso-ansi-language: EN-US mso-fareast-language: EN-US mso-bidi-language: FA mso-no-proof: yes mso-bidi-font-size: 11.0pt mso-bidi-font-family: Lotus">OP</span><span dir="rtl"></span><span style="FONT-FAMILY: Lotus FONT-SIZE: 11pt mso-fareast-font-family: 'Times New Roman' mso-ansi-language: EN-US mso-fareast-language: EN-US mso-bidi-language: FA mso-ansi-font-size: 9.0pt mso-ascii-font-family: 'Times New Roman Bold' mso-hansi-font-family: 'Times New Roman Bold' mso-no-proof: yes" dir="rtl" lang="FA"><span dir="rtl"></span>) و فسفر باقی‌مانده (</span><span style="FONT-FAMILY: "Times New Roman Bold","serif" FONT-SIZE: 9pt mso-fareast-font-family: 'Times New Roman' mso-ansi-language: EN-US mso-fareast-language: EN-US mso-bidi-language: FA mso-no-proof: yes mso-bidi-font-size: 11.0pt mso-bidi-font-family: Lotus">Residual-P</span><span dir="rtl"></span></strong><span style="FONT-FAMILY: Lotus FONT-SIZE: 11pt mso-fareast-font-family: 'Times New Roman' mso-ansi-language: EN-US mso-fareast-language: EN-US mso-bidi-language: FA mso-ansi-font-size: 9.0pt mso-ascii-font-family: 'Times New Roman Bold' mso-hansi-font-family: 'Times New Roman Bold' mso-no-proof: yes" dir="rtl" lang="FA"><span dir="rtl"></span><strong>) نیز تعیین شدند. فعالیت آنزیم فسفاتاز قلیایی نیز مورد ارزیابی قرار گرفت. سپس، به منظور بررسی شاخص­های گیاه لوبیا، یک کشت گلدانی در قالب طرح کاملاً تصادفی در سه تکرار در ده نوع خاک اجرا شد. نتایج نشان داد که فراوانی اجزای فسفر عمدتاً به‌صورت زیر کاهش یافت:<br></strong></span><strong><span style="FONT-FAMILY: "Times New Roman Bold","serif" FONT-SIZE: 9pt mso-fareast-font-family: 'Times New Roman' mso-ansi-language: EN-US mso-fareast-language: EN-US mso-bidi-language: FA mso-no-proof: yes mso-bidi-font-size: 11.0pt mso-bidi-font-family: Lotus">HCl-P</span><span dir="rtl"></span><span style="FONT-FAMILY: Lotus FONT-SIZE: 11pt mso-fareast-font-family: 'Times New Roman' mso-ansi-language: EN-US mso-fareast-language: EN-US mso-bidi-language: FA mso-ansi-font-size: 9.0pt mso-ascii-font-family: 'Times New Roman Bold' mso-hansi-font-family: 'Times New Roman Bold' mso-no-proof: yes" dir="rtl" lang="FA"><span dir="rtl"></span>></span><span style="FONT-FAMILY: "Times New Roman Bold","serif" FONT-SIZE: 9pt mso-fareast-font-family: 'Times New Roman' mso-ansi-language: EN-US mso-fareast-language: EN-US mso-bidi-language: FA mso-no-proof: yes mso-bidi-font-size: 11.0pt mso-bidi-font-family: Lotus">Residual-P</span><span dir="rtl"></span><span style="FONT-FAMILY: Lotus FONT-SIZE: 11pt mso-fareast-font-family: 'Times New Roman' mso-ansi-language: EN-US mso-fareast-language: EN-US mso-bidi-language: FA mso-ansi-font-size: 9.0pt mso-ascii-font-family: 'Times New Roman Bold' mso-hansi-font-family: 'Times New Roman Bold' mso-no-proof: yes" dir="rtl" lang="FA"><span dir="rtl"></span> ></span><span style="FONT-FAMILY: "Times New Roman Bold","serif" FONT-SIZE: 9pt mso-fareast-font-family: 'Times New Roman' mso-ansi-language: EN-US mso-fareast-language: EN-US mso-bidi-language: AR-SA mso-no-proof: yes mso-bidi-font-size: 11.0pt mso-bidi-font-family: Lotus">NaOH+CB]-P</span><span dir="rtl"></span><span style="FONT-FAMILY: Lotus FONT-SIZE: 11pt mso-fareast-font-family: 'Times New Roman' mso-ansi-language: EN-US mso-fareast-language: EN-US mso-bidi-language: AR-SA mso-ansi-font-size: 9.0pt mso-ascii-font-family: 'Times New Roman Bold' mso-hansi-font-family: 'Times New Roman Bold' mso-no-proof: yes" dir="rtl" lang="AR-SA"><span dir="rtl"></span>]</span><span style="FONT-FAMILY: Lotus FONT-SIZE: 11pt mso-fareast-font-family: 'Times New Roman' mso-ansi-language: EN-US mso-fareast-language: EN-US mso-bidi-language: FA mso-ansi-font-size: 9.0pt mso-ascii-font-family: 'Times New Roman Bold' mso-hansi-font-family: 'Times New Roman Bold' mso-no-proof: yes" dir="rtl" lang="FA"> ></span><span style="FONT-FAMILY: "Times New Roman Bold","serif" FONT-SIZE: 9pt mso-fareast-font-family: 'Times New Roman' mso-ansi-language: EN-US mso-fareast-language: EN-US mso-bidi-language: FA mso-no-proof: yes mso-bidi-font-size: 11.0pt mso-bidi-font-family: Lotus">OP</span><span dir="rtl"></span><span style="FONT-FAMILY: Lotus FONT-SIZE: 11pt mso-fareast-font-family: 'Times New Roman' mso-ansi-language: EN-US mso-fareast-language: EN-US mso-bidi-language: FA mso-ansi-font-size: 9.0pt mso-ascii-font-family: 'Times New Roman Bold' mso-hansi-font-family: 'Times New Roman Bold' mso-no-proof: yes" dir="rtl" lang="FA"><span dir="rtl"></span> ></span><span style="FONT-FAMILY: "Times New Roman Bold","serif" FONT-SIZE: 9pt mso-fareast-font-family: 'Times New Roman' mso-ansi-language: EN-US mso-fareast-language: EN-US mso-bidi-language: FA mso-no-proof: yes mso-bidi-font-size: 11.0pt mso-bidi-font-family: Lotus">CBD-P</span><span dir="rtl"></span><span style="FONT-FAMILY: Lotus FONT-SIZE: 11pt mso-fareast-font-family: 'Times New Roman' mso-ansi-language: EN-US mso-fareast-language: EN-US mso-bidi-language: FA mso-ansi-font-size: 9.0pt mso-ascii-font-family: 'Times New Roman Bold' mso-hansi-font-family: 'Times New Roman Bold' mso-no-proof: yes" dir="rtl" lang="FA"><span dir="rtl"></span>. هم‌چنین، همبستگی معنی­داری بین فعالیت آنزیم فسفاتاز قلیایی با</span><span dir="ltr"></span><span style="FONT-FAMILY: "Times New Roman Bold","serif" FONT-SIZE: 9pt mso-fareast-font-family: 'Times New Roman' mso-ansi-language: EN-US mso-fareast-language: EN-US mso-bidi-language: FA mso-no-proof: yes mso-bidi-font-size: 11.0pt mso-bidi-font-family: Lotus" lang="FA"><span dir="ltr"></span> </span><span style="FONT-FAMILY: Lotus FONT-SIZE: 11pt mso-fareast-font-family: 'Times New Roman' mso-ansi-language: EN-US mso-fareast-language: EN-US mso-bidi-language: FA mso-ansi-font-size: 9.0pt mso-ascii-font-family: 'Times New Roman Bold' mso-hansi-font-family: 'Times New Roman Bold' mso-no-proof: yes" dir="rtl" lang="FA">جزء </span><span style="FONT-FAMILY: "Times New Roman Bold","serif" FONT-SIZE: 9pt mso-fareast-font-family: 'Times New Roman' mso-ansi-language: EN-US mso-fareast-language: EN-US mso-bidi-language: FA mso-no-proof: yes mso-bidi-font-size: 11.0pt mso-bidi-font-family: Lotus">CBD-P</span><span dir="rtl"></span><span style="FONT-FAMILY: Lotus FONT-SIZE: 11pt mso-fareast-font-family: 'Times New Roman' mso-ansi-language: EN-US mso-fareast-language: EN-US mso-bidi-language: FA mso-ansi-font-size: 9.0pt mso-ascii-font-family: 'Times New Roman Bold' mso-hansi-font-family: 'Times New Roman Bold' mso-no-proof: yes" dir="rtl" lang="FA"><span dir="rtl"></span>، فسفر آلی و فسفر کل وجود داشت. به‌علاوه، اجزای </span><span style="FONT-FAMILY: "Times New Roman Bold","serif" FONT-SIZE: 9pt mso-fareast-font-family: 'Times New Roman' mso-ansi-language: EN-US mso-fareast-language: EN-US mso-bidi-language: AR-SA mso-no-proof: yes mso-bidi-font-size: 11.0pt mso-bidi-font-family: Lotus">NaOH+CB]-P</span><span dir="rtl"></span><span style="FONT-FAMILY: Lotus FONT-SIZE: 11pt mso-fareast-font-family: 'Times New Roman' mso-ansi-language: EN-US mso-fareast-language: EN-US mso-bidi-language: AR-SA mso-ansi-font-size: 9.0pt mso-ascii-font-family: 'Times New Roman Bold' mso-hansi-font-family: 'Times New Roman Bold' mso-no-proof: yes" dir="rtl" lang="AR-SA"><span dir="rtl"></span>]</span><span style="FONT-FAMILY: Lotus FONT-SIZE: 11pt mso-fareast-font-family: 'Times New Roman' mso-ansi-language: EN-US mso-fareast-language: EN-US mso-bidi-language: FA mso-ansi-font-size: 9.0pt mso-ascii-font-family: 'Times New Roman Bold' mso-hansi-font-family: 'Times New Roman Bold' mso-no-proof: yes" dir="rtl" lang="FA"> و </span><span style="FONT-FAMILY: "Times New Roman Bold","serif" FONT-SIZE: 9pt mso-fareast-font-family: 'Times New Roman' mso-ansi-language: EN-US mso-fareast-language: EN-US mso-bidi-language: AR-SA mso-no-proof: yes mso-bidi-font-size: 11.0pt mso-bidi-font-family: Lotus">HCl-P</span><span dir="rtl"></span><span style="FONT-FAMILY: Lotus FONT-SIZE: 11pt mso-fareast-font-family: 'Times New Roman' mso-ansi-language: EN-US mso-fareast-language: EN-US mso-bidi-language: FA mso-ansi-font-size: 9.0pt mso-ascii-font-family: 'Times New Roman Bold' mso-hansi-font-family: 'Times New Roman Bold' mso-no-proof: yes" dir="rtl" lang="FA"><span dir="rtl"></span> همبستگی معنی­داری با فسفر جذب شده توسط بخش هوایی لوبیا داشتند. به‏طورکلی، </span><span style="FONT-FAMILY: Lotus FONT-SIZE: 11pt mso-fareast-font-family: 'Times New Roman' mso-ansi-language: EN-US mso-fareast-language: EN-US mso-bidi-language: AR-SA mso-ansi-font-size: 9.0pt mso-ascii-font-family: 'Times New Roman Bold' mso-hansi-font-family: 'Times New Roman Bold' mso-no-proof: yes" dir="rtl" lang="AR-SA">نتایج این تحقیق نشان داد که </span><span style="FONT-FAMILY: Lotus FONT-SIZE: 11pt mso-fareast-font-family: 'Times New Roman' mso-ansi-language: EN-US mso-fareast-language: EN-US mso-bidi-language: FA mso-ansi-font-size: 9.0pt mso-ascii-font-family: 'Times New Roman Bold' mso-hansi-font-family: 'Times New Roman Bold' mso-no-proof: yes" dir="rtl" lang="FA">روش جزءبندی مرحله­ای فسفر می­تواند ابزار ارزشمندی در تعیین وضعیت فسفر خاک­ها و قابلیت دسترسی این عنصر برای گیاهان در خاک­های تیمار شده با لجن فاضلاب شهری فراهم کند</span><span style="FONT-FAMILY: Lotus FONT-SIZE: 11pt mso-fareast-font-family: 'Times New Roman' mso-ansi-language: EN-US mso-fareast-language: EN-US mso-bidi-language: AR-SA mso-ansi-font-size: 9.0pt mso-ascii-font-family: 'Times New Roman Bold' mso-hansi-font-family: 'Times New Roman Bold' mso-no-proof: yes" dir="rtl" lang="AR-SA">.</span></strong></p> <span class="hps"><span style="FONT-FAMILY: "Times New Roman","serif" FONT-SIZE: 10pt mso-fareast-font-family: 'Times New Roman' mso-ansi-language: EN-US mso-fareast-language: EN-US mso-bidi-language: FA">T</span></span><span style="FONT-FAMILY: "Times New Roman","serif" FONT-SIZE: 10pt mso-fareast-font-family: 'Times New Roman' mso-ansi-language: EN-US mso-fareast-language: EN-US mso-bidi-language: FA">o evaluate the relationship of various P fractions and alkaline phosphatase activity with bean indices growing in 10 calcareous soils, amended with municipal sewage sludge from Chaharmahal-Va-Bakhtiari province, a greenhouse research was carried out. Soil samples were incubated for one month with sludge at a rate equivalent to 1% (w/w). <font size="2"><font face="Times New Roman"><span class="hps">Then, the P</span> <span class="hps">fractions, including P adsorbed by Fe and Al oxides (</span><span dir="rtl"></span><span dir="rtl" lang="FA"><span dir="rtl"></span>]</span></font></font></span><span style="FONT-FAMILY: "Times New Roman","serif" FONT-SIZE: 10pt mso-fareast-font-family: 'Times New Roman' mso-ansi-language: EN-US mso-fareast-language: EN-US mso-bidi-language: AR-SA">NaOH+CB]-P</span><span class="hps"><span style="FONT-FAMILY: "Times New Roman","serif" FONT-SIZE: 10pt mso-fareast-font-family: 'Times New Roman' mso-ansi-language: EN-US mso-fareast-language: EN-US mso-bidi-language: FA">), occluded P (</span></span><span style="FONT-FAMILY: "Times New Roman","serif" FONT-SIZE: 10pt mso-fareast-font-family: 'Times New Roman' mso-ansi-language: EN-US mso-fareast-language: EN-US mso-bidi-language: FA">CBD-P<font size="2"><font face="Times New Roman"><span class="hps">) and P absorbed by Ca (</span>HCl-P<span class="hps">),</span> <span class="hps">were determined by</span> <span class="hps">Olsen</span> <span class="hps">and</span> <span class="hps">Summers'</span> sequential fractionation procedure. Furthermore, total P, organic P and residual P were determined. Also, alkaline phosphatase activity was measured. A <span class="hps">pot experiment in</span> a <span class="hps">completely randomized design</span> <span class="hps">with three</span> <span class="hps">replications in the ten soils was done to</span> <span class="hps">evaluate the bean</span> <span class="hps">plant</span> <span class="hps">indices</span>. <span class="hps">The results showed that the</span> <span class="hps">amount</span> <span class="hps">of</span> P fractions<span class="hps"> decreased in the following order: </span>HCl-P>residual-P><span dir="rtl"></span><span dir="rtl" lang="FA"><span dir="rtl"></span>]</span></font></font></span><span style="FONT-FAMILY: "Times New Roman","serif" FONT-SIZE: 10pt mso-fareast-font-family: 'Times New Roman' mso-ansi-language: EN-US mso-fareast-language: EN-US mso-bidi-language: AR-SA">NaOH+CB]-P</span><span dir="rtl"></span><span style="FONT-FAMILY: "Times New Roman","serif" FONT-SIZE: 10pt mso-fareast-font-family: 'Times New Roman' mso-ansi-language: EN-US mso-fareast-language: EN-US mso-bidi-language: FA" dir="rtl"><span dir="rtl"></span> </span><span dir="ltr"></span><span style="FONT-FAMILY: "Times New Roman","serif" FONT-SIZE: 10pt mso-fareast-font-family: 'Times New Roman' mso-ansi-language: EN-US mso-fareast-language: EN-US mso-bidi-language: FA"><span dir="ltr"></span>> OP>CBD-P. The results also indicated that alkaline phosphatase activity was significantly correlated with CBD-P fraction, organic P and total P. In addition, significant correlations were found between ([</span><span style="FONT-FAMILY: "Times New Roman","serif" FONT-SIZE: 10pt mso-fareast-font-family: 'Times New Roman' mso-ansi-language: EN-US mso-fareast-language: EN-US mso-bidi-language: AR-SA">NaOH+CB]-P)<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span><font size="2" face="Times New Roman"> </font></span>and HCl-P</span><span style="FONT-FAMILY: "Times New Roman","serif" FONT-SIZE: 10pt mso-fareast-font-family: 'Times New Roman' mso-ansi-language: EN-US mso-fareast-language: EN-US mso-bidi-language: FA"> and plant shoots. In general, the results of this research showed that P fractionation method appears to be a powerful tool to identify the P status and availability in the soils amended with sewage sludge.</span> : لجن فاضلاب, جزء‌بندی فسفر, فسفاتاز قلیایی Sewage sludge, P fractionation, Alkaline phosphatase 39 50 http://jspi.iut.ac.ir/browse.php?a_code=A-10-1-5&slc_lang=fa&sid=1 T. Raeisi طاهره رئیسی taraiesi@gmail.com Yes Shahrekord Univ., Shahrekord, Iran دانشکده کشاورزی، دانشگاه شهرکرد A. R. Hosseinpur علیرضا حسین‌پور No Shahrekord Univ., Shahrekord, Iran دانشکده کشاورزی، دانشگاه شهرکرد