1- Department of Horticultural Science, Faculty of Agriculture and Natural Resources, Ardakan University, Ardakan, Iran
2- Department of Horticultural Science, Faculty of Agriculture and Natural Resources, Ardakan University, Ardakan, Iran , mdehestani@ardakan.ac.ir
Abstract: (291 Views)
Background and Objective: Narcissus tazetta L. cv. Shahla is an important ornamental and medicinal bulbous plant valued for its fragrance and cut flower quality. This study aimed to investigate the effects of natural biostimulants, sodium nitroprusside, and melatonin on the morphophysiological and biochemical characteristics of Narcissus tazetta cv. Shahla.
Methods: A field experiment was conducted in a randomized complete blocks design with nine treatments and three replications to evaluate the effects of natural biostimulants on growth and flower quality of N. tazetta. Treatments included moringa leaf extract (2.5 and 5.0%), seaweed extract (20 and 40 g L⁻¹), sodium nitroprusside (50 and 100 mM), and melatonin (0.1 and 0.5 mM), applied twice as foliar sprays. The first application was made about 30 days after planting, when the plants were 20 to 25 cm tall, and the second application was 15 days after the first foliar spray.
Results: The 0.1 mM melatonin treatment produced the highest leaf length (365.2 mm) and shoot fresh weight (29.6 g plant⁻¹). The 5% moringa extract significantly increased leaf width (45.3 mm), root fresh weight (40.6 g plant⁻¹), and vase life (12 days) compared to 7 days in the control. The lowest electrolyte leakage (8.75%) was recorded in 20 g L⁻¹ seaweed extract, whereas the control showed the highest value (94.8%). The highest total protein content (4.06 mg g⁻¹ FW), peroxidase activity (2.12 ΔOD min⁻¹ mg⁻¹ protein), and catalase activity (3.24 ΔOD min⁻¹ mg⁻¹ protein) were obtained in the 100 mM sodium nitroprusside treatment.
Conclusion: Generally, moringa leaf extract and sodium nitroprusside were the most effective treatments in enhancing quantitative and qualitative traits of N. tazetta, supporting their potential application in sustainable ornamental production systems.